Isolation and characterization of plant growth promoting rhizobacteria (pgpr) from agricultural field and their potential role on germination and growth of spinach (spinacea oleracea l.) plants

Ananya RoyChowdhury, Anindita Bagchi and Chandan Sengupta

Various chemical fertilizers are used in different doses for crop improvement purpose in agriculture. But for several side effects of agrochemicals there is an increased concern on the co-operative activities between plants and the rhizospheric microbes. So, now a days the PGPR (Plant Growth Promoting Rhizobacteria) application opening a new gateway to solve these problems. The PGPRs not only can improve soil fertility but also enhance plant growth. In this article we are going to focus on the potential role of PGPR, isolated from the agricultural land. Here we examined the growth of spinach plant with four isolated PGPR strains in the Kalyani University campus. In all studied cases, we observed that different growth parameters in the uninoculated spinach (Spinacia oleracea L.) plants (i.e-without PGPR) were reduced as compared with treated plants. Absence of PGPR activities were assessed by observing different growth parameters like low germination %, reduced growth and poor rooting and low chlorophyll content. Inoculation of PGPR in spinach plant enhanced the different growth parameters as compared with uninoculated plants. Our present study conducted with 4 out of 20 different bacterial isolates and observed results indicated that SIN 1,SIN 2,SIN 3 ,SIN 4 (named according to the place and priority of isolate such as, Sample Isolate Nadia 1) were very effective to promote plant growth.

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