In vitro clonal micropropagation of hylocereus undatus :a palatable dragon fruit

Author: 
Ravindra Babu Potti, Snehasri Motamarr, Manasa roopa and Anitha Paladugu

An in vitro micropropagation method was developed for Hylocereus undatus, belonging to the family Cactaceae, a perennial epiphyte. It has anti-proliferative properties and is rich in antioxidants, Flavonoids, Polyphenol, and pigment, betacyanin. H.undatus, also known as Pitaya has gained interest as ornament than fruit crop. The small pitaya seeds are less viable in stored condition. In the present study efficient micropropagation protocol was investigated to meet the increasing demand of the fruit crop. Micropropagation was carried out in 2 phases, shoot induction and root induction. Shoot explants (sectioned areoles, without removing thorns)were cultured on MS media supplemented with combinations of phyto-hormones BAP0.4, IAA 0.1,NAA 0.1 ,KN 0.2 which induced highest shoots after sixty days of incubation. Roots were induced in MS media supplemented with IAA1.6 and incubated for 60 days. These plantlets were successfully acclimatized in vermicompost and peat moss (1:1) with more than 82 % survival rate. Further, these plants showed similar growth in height and stem diameter grown that were simultaneously planted in the pot.
Abbreviations: 6-BAP, 6-benzylaminopurine; 2,4-D, 2,4-dichlorophenoxyacetic acid; IAA, indole-3-acetic acid; Kn, kinetin; NAA, 1-naphthaleneacetic acid; MS, Murashige and Skoog medium

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