Preliminary phytochemical screening and antibacterial activitiy of acalypha indica and euphorbia hirta of family euphorbiaceae against some pathogenic organisms

Poornima, R and Prabakaran, G

Plant and plant-based products are the base for many of the modern pharmaceuticals that we use today for various alignments. The objective of the study was to determine the bioactive chemical constituents and to evaluate the extracts of Acalypha indica and Euphorbia hirta for in vitro antimicrobial activities. Preliminary phytochemical analysis of the crude extracts of Acalypha indica revealed the presence of alkaloids, flavonoids, phenolic compounds and saponins. Euphorbia hirta revealed the presence of anthroquinones, flavonoids, phenolic compounds and saponins.The antimicrobial activity was determined by disc diffusion technique using eight reference bacterial strains vi,: Bacillus subtilis, Escherichia coli, Enterococcus feacials, Pseudomonas aeroginosa, Staphylococcus aureus, Staphylococcus epidermis, Klebsiella pneumonia and Proteus vulgaris. Ethyl acetate extract of Acalypha indica exerted mean zone of inhibition in the range of 10-23mm. Euphorbia hirta exhibited zone of inhibition from 18-31mm for the solvent ethyl acetate. Methanol, ethanol, diethyl ether and distilled water extract of Acalypha indica were bactericidal against tested bacteria. The MIC and MBC of both the plant extracts ranged between 30-90mg/ml. The antimicrobial activities of these extracts is currently being undertaken to identify the secondary metabolites responsible for the bioactivity will be studied further